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US 2021/0230772 A1Published application
HLA HOMOZYGOUS INDUCED PLURIPOTENT STEM CELL (iPSC) LIBRARIES
AssigneeNew York Blood Center, Inc.
InventorPablo Rubinstein
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Abstract
The present specification provides libraries of HLA homozygous induced pluripotent cell lines.
Claims26
A library of HLA homozygous induced pluripotent stem cell (iPS) lines, the library comprising:
- at least 20 iPS cell lines homozygous for alleles at HLA-A, -B, and -DR loci, each cell line expressing a different homozygous haplotype;
- wherein each of the iPS cell lines has been reprogrammed into pluripotentiality by means of non-endogenous transcription factors.
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A method of producing a library of HLA homozygous iPSC comprising:
- screening cord blood units donated to a cord blood repository for:
- a) genetic, cytogenetic and other genomic and chromosomal abnormalities
- b) history and markers of exposure to infectious diseases
- c) homozygosity for all of HLA-A, -B, -C, and -Dr loci;
- isolating white blood cells from the cord blood units negative for genetic defects, exposure to infectious diseases, and transposons, and homozygous for HLA-A, -B, -C, and -Dr, causing the white blood cells to express Oct-4, Sox-2, Klf-4, and c-Myc to form iPS cells;
- culturing and cloning the iPSC to produce a population of cloned iPS cells homozygous for their HLA-A, -B, -C, and -DR haplotypes;
- characterizing the genotype of the iPSC by typing of HLA markers beyond the A,B,C and DR loci to include additional class I, II and III genes;
- screening the cells from iPSC clones for the presence of chromosomal aberrations and evidences of genetic instability and
- cryoprotecting and storing the cloned population of iPSC.
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Dependencies are inferred from the claim text.