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US 2021/0230692 A1Published application
Surface capture of targets
OwnerEvan Daugharthy, Richard Terryinventors
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Abstract
Provided herein are methods and systems for transfer target molecules to a surface, such as a planar surface. The transferred target molecules can be used for downstream applications, such as sequence identification.
Claims29
A method for analyzing nucleic acid molecules in a biological sample, comprising:
- (a) providing a biological sample to an array comprising a plurality of capture probes;
- (b) applying a pressure field to the biological sample to direct a plurality of nucleic acid molecules in the biological sample toward the array;
- (c) using a subset of the plurality of capture probes to capture a subset of the plurality of nucleic acid molecules, thereby immobilizing the subset of the plurality of nucleic acid molecules to the array;
- (d) identifying sequences and positions of the subset of the plurality of nucleic acid molecules immobilized to the array;
- and
- (e) using the positions identified in step (d) to identify the sequences as originating from corresponding positions in the biological sample.
3 more elements
A method for analyzing nucleic acid molecules in a biological sample, comprising:
- (a) providing a biological sample to an array having a plurality of capture probes under conditions sufficient to direct a plurality of nucleic acid molecules from the biological sample toward the array comprising the plurality of capture probes, wherein the plurality of nucleic acid molecules are directed toward the array using a pressure field at a rate that is greater than a rate of diffusion or gravity-assisted flow of the plurality of nucleic acid molecules in the biological sample;
- (b) using a subset of the plurality of capture probes to capture a subset of the plurality of nucleic acid molecules, thereby immobilizing the subset of the plurality of nucleic acid molecules to the array;
- (c) identifying sequences and positions of the subset of the plurality of nucleic acid molecules immobilized to the array;
- and
- using the positions identified in (c) to identify the sequences as originating from corresponding positions within the biological sample.
2 more elements
Dependencies are inferred from the claim text.