Back to results
US 2021/0230688 A1Published application
Analyte detection
OwnerAlexander Johnson-Buckinventor
Life of this patent
Estimating the term…
Abstract
Provided herein is technology relating to analyte detection and particularly, but not exclusively, to compositions, methods, systems, and kits for high-specificity detection of analytes using molecular probes that integrate cumulative repeated binding of probes to the same analyte molecule.
Claims23
A composition for detecting an analyte in a sample, said composition comprising:
- an integrator probe that is capable of stably associating with an analyte;
- and
- a tally probe comprising a label and that is capable of directly or indirectly associating with said analyte,
- wherein irreversible transfer of said label from said tally probe to said integrator probe occurs more rapidly and/or more efficiently when said tally probe and said integrator probe are both associated with said analyte than when said tally probe and/or said integrator probe is/are dissociated from said analyte.
1 more element
9-11. (canceled)
(canceled)
A system for detecting an analyte in a sample, said system comprising:
- an integrator probe that is capable of stably associating with an analyte;
- and
- a tally probe comprising a label and that is capable of directly or indirectly associating with said analyte,
- wherein irreversible transfer of said label from said tally probe to said integrator probe occurs more rapidly and/or more efficiently when said tally probe and said integrator probe are both associated with said analyte than when said tally probe and/or said integrator probe is/are dissociated from said analyte;
- and
- a detection component configured to detect and/or quantify a signal produced by an integrator probe comprising multiple labels.
3 more elements
23-43. (canceled)
A method of detecting and/or quantifying an analyte, said method comprising:
- providing a sample comprising an analyte;
- providing an integrator probe that is capable of stably associating with an analyte;
- and
- providing a tally probe comprising a label and that is capable of directly or indirectly associating with said analyte.
1 more element
45-73. (canceled)
Dependencies are inferred from the claim text.