Methods and compositions for incorporating nucleotides
AssigneeQiagen Sciences, LLC
InventorsSteven Gordon, Jerzy Olejnik
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Abstract
The invention provides methods and compositions, including, without limitation, algorithms, computer readable Media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.
Claims9
1-6. (canceled)
A method for determining an identity of a nucleotide at a position, comprising:
- a) providing nucleic acid, polymerase, and a mixture of labeled and non-labeled reversible terminators, said labeled reversible terminators comprising a label linked to a nucleotide analogue;
- b) exposing said nucleic acid, said polymerase, and said mixture to extension conditions for 10 minutes or less, wherein a first nucleotide analogue from said mixture is incorporated into said nucleic acid at a position;
- c) detecting the label of said incorporated first nucleotide analogue;
- and
- d) determining the identity of the incorporated nucleotide at said position.
Dependencies are inferred from the claim text.