US 2021/0230680 A1 · Modified Nucleobases with Uniform H-Bonding Interactions, Homo- and Hetero-Basepair Bias, and Mismatch Discrimination

Abstract

Described herein are divalent nucleobases that each binds two nucleic acid strands, matched or mismatched when incorporated into a nucleic acid or nucleic acid analog backbone, such as in a γ-peptide nucleic acid (γPNA). Also provided are genetic recognition reagents comprising one or more of the divalent nucleobases and a nucleic acid or nucleic acid analog backbone, such as a γPNA backbone. Uses for the divalent nucleobases and monomers and genetic recognition reagents containing the divalent nucleobases also are provided.

Claims50

Dependent claims

A genetic recognition reagent comprising a plurality of nucleobase moieties attached to a nucleic acid or nucleic acid analog backbone, in which at least one nucleobase moiety is:

wherein,
X1 is ═O, ═S, ═Se, or CH3;
X2 is H, CH3, CN, NC, N3, C(O)OH, or C(O)NH2;
X3 is O or S;
X4 is H, C(O)CH3, or C(O)OCH3;
and
Y is N or CH,
wherein in (1), when X1 and X3 are O, X2 is not H or methyl.

5 more elements

Dependencies are inferred from the claim text.

Modified Nucleobases with Uniform H-Bonding Interactions, Homo- and Hetero-Basepair Bias, and Mismatch Discrimination

Life of this patent

Modified Nucleobases with Uniform H-Bonding Interactions, Homo- and Hetero-Basepair Bias, and Mismatch Discrimination

Life of this patent

Abstract

Claims50