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US 2021/0230677 A1Published application
Application of cas protein, method for detecting target nucleic acid molecule and kit
AssigneeShanghai Tolo Biotechnology Company Limited
InventorsJin Wang, Qiuxiang Cheng, Shiyuan Li, Xiaoyan Li, Linxian Li
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Abstract
The present invention provides a use of a Cas protein, and a method and a kit for detecting target nucleic acid molecules. The method for detecting target nucleic acid molecules comprises adding a guide RNA, a Cas12a, and a nucleic acid probe into a reaction system containing target nucleic acid molecules to be detected, and detecting it after the reaction is completed.
Claims15
-21. (canceled)
A method for detecting target nucleic acid molecules comprising steps of:
- (a) providing a mixture including:
- a Cas12 protein having collateral cleavage activity;
- a guide RNA operative with the Cas12 protein;
- a detectably labeled nucleic acid probe susceptible to the collateral cleavage activity;
- a buffer;
- and
- a system in which target nucleic acid molecules are to be detected;
- and
- (b) detecting cleavage of the nucleic acid probe.
6 more elements
A combination comprising:
- a Cas12 protein having collateral cleavage activity;
- a guide RNA operative with the Cas12 protein and directed to a sequence in a target nucleic acid;
- a detectably labeled nucleic acid probe;
- and
- a buffer.
2 more elements
Dependencies are inferred from the claim text.