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US 2021/0230676 A1Published application
Sequential Staining for Multiplex Analyses of Tissues and Cells
AssigneeRush University Medical Center
InventorsVineet GUPTA, Anugraha RAJAGOPALAN
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Abstract
Compositions and methods are provided including a plurality of analyte detection agents, each analyte detection agent includes a labile tag operatively coupled to the analyte detection agent, each labile tag includes a signal that is different from each other labile tag and each analyte detection agent targeting a different analyte.
Claims25
A composition comprising:
- a plurality of analyte detection agents, each analyte detection agent comprising a labile tag operatively coupled to the analyte detection agent, each labile tag comprising a signal that is different from each other labile tag and each analyte detection agent targeting a different analyte;
- wherein each labile tag is removable or quenchable without destroying a sample to which the plurality of analyte detection agents is applied and allows for further analysis of the sample with a second plurality of analyte detection agents.
A method for analyzing a sample, the method comprising:
- (a) labeling a sample with a plurality of analyte detection agents, each analyte detection agent comprising a labile tag operatively coupled to the analyte detection agent, each labile tag comprising a signal that is different from each other labile tag and each analyte detection agent targeting a different analyte;
- (b) detecting the signal generated by each of the plurality of analyte detection agents on the sample;
- (c) removing the signal from each analyte detection agent;
- and
- (d) repeating steps a-c.
2 more elements
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Dependencies are inferred from the claim text.