Assays for detecting multiple tick-borne pathogens
AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorsADALBERTO A. PEREZ de LEON, MARIA D. ESTEVE-GASENT, PAMELA J. FERRO, JOSEPH J. MODARELLI
Life of this patent
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Abstract
Kits, methods, and material for use in detecting tick-borne microorganism(s) are provided herein. One is able to detect DNA from several different species of microorganisms from samples (bodily fluid, tissue, etc.) from ticks and/or mammals to determine if one or more of the microorganisms has infected the tick and/or mammal. The assay uses PCR techniques with a multiple primers and probes. The microorganisms can be in the Borrelial Layer, the Rickettsiales Layer, and/or the Babesial Layer.
Claims20
A kit for detecting Borrelia spp., Rickettsia-like bacteria, or Babesia spp. in a sample comprising at least one primer set for detecting said Borrelia spp., Rickettsia-like bacteria, or Babesia spp., an amplification control DNA, instructions for using said at least one primer set and said amplification control plasmid, optionally PCR reagents, and optionally reagents for isolating DNA from a sample.
A method for detecting at least one tick-borne pathogen in a sample from an animal comprising
- a) optionally, isolating DNA from said sample to provided isolated DNA;
- b) contacting said isolated DNA with a plurality of primer sets and DNA polymerase to provide a first layer PCR mix, wherein said plurality of primer sets detect at least one tick-borne pathogen;
- c) performing PCR on said first layer PCR mix to generate at least one amplicon;
- and
- d) determining the identify of said generated at least one amplicon, wherein said at least one amplicon is specific for detecting said at least one tick-borne pathogen in said sample.
Dependencies are inferred from the claim text.