Detection of Reversal of an Anticoagulant Using a Clotting Assay

A method for detecting reversal of an anticoagulant at a therapeutically relevant amount or higher in a patient having an anticoagulant, the method comprising:

• (a) subjecting a sample of a control blood component known to lack the anticoagulant to a clotting assay in the presence of an ecarin reagent to obtain a control ecarin clotting measurement;
• (b) subjecting a sample of the control blood component known to lack the anticoagulant to a clotting assay in the presence of a Factor Xa reagent to obtain a control Factor Xa clotting measurement;
• (c) subjecting a sample of a blood component from a patient suspected to contain a reversal agent to a clotting assay in the presence of the ecarin reagent to obtain a patient ecarin clotting measurement;
• (d) subjecting a sample of the blood component from the patient suspected to contain the reversal agent to a clotting assay in the presence of the Factor Xa reagent to obtain a patient Factor Xa clotting measurement;
• and
• (e) comparing the control ecarin clotting measurement to the patient ecarin clotting measurement and comparing the control Factor Xa clotting measurement to the patient Factor Xa measurement,
• wherein the patient ecarin clotting measurement less than or equal to the control ecarin clotting measurement and/or the patient Factor Xa clotting measurement less than or equal to the control Factor Xa clotting measurement identifies the blood component from the patient as having a reversal agent that has reversed the anticoagulation activity of the anticoagulant in the patient.