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US 2021/0230658 A1Published application
Methods of protein production
AssigneeGenentech, Inc.
InventorsInn Huam Yuk, Bradley Richard Snedecor, Dana Christian Andersen
Life of this patent
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Abstract
A method of reducing glycation of an amino acid in a protein produced by a host cell in cell culture medium is disclosed.
Claims20
A method of producing a polypeptide with reduced glycation comprising:
- (a) culturing host cells expressing the polypeptide in a cell growth phase,
- (b) thereafter culturing the cells in a polypeptide production phase in a cell culture medium comprising at least one reducing sugar in which the total reducing sugar concentration is maintained at about 5 g/L or less by a continuous reducing sugar feed or a continuous nutrient feed comprising at least one reducing sugar, and
- (c) thereafter harvesting the polypeptide from the cell culture.
Dependencies are inferred from the claim text.