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US 2021/0230656 A1Published application
Production of oligosaccharides
AssigneeDsm IP Assets B.V.
InventorsArthur Maurits Christiaan JANSE, Michael Benjamin JOHNSON
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Abstract
A method for producing and purifying human milk oligosaccharides (HMOs) is provided. The method includes fermentation of a genetically modified microbial organism, preferably a genetically modified yeast strain, and downstream processing of the fermentation product using one or more of an enzymatic treatment, filtration, and a simulated moving bed (SMB) chromatography step. Use of the resulting HMO in food or feed applications, preferably in infant food and/or formula is also provided.
Claims32
A method for the production of one or more human milk oligosaccharides (HMOs) comprising:
- a) fermentation of a microbial organism that has been genetically modified to produce one or more HMOs in a suitable fermentation medium to form a fermentation product;
- b) enzymatic treatment of the fermentation product;
- c) removal of the biomass from the fermentation product;
- d) ultrafiltration;
- e) nanofiltration;
- and
- f) a column chromatography step.
4 more elements
Dependencies are inferred from the claim text.