Systems, methods and compositions for the generation novel high yielding waxes from microalgae
AssigneePebble Labs USA Inc.
InventorsRichard Sayre, Tawanda Zidenga, Stephanie Willette
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Abstract
The invention relates to the chemical synthesis of waxes. Specifically, the invention relates to systems and methods for the high-yield production of novel and high-value waxes in genetically-modified algae-based systems as a replacement for petroleum-based products.
Claims31
A method of the wax biosynthesis comprising the step of transforming an algal cell with one or more polynucleotide sequences operably linked to a promoter that expresses a heterologous fatty acyl-CoA reductase (FAR), and a heterologous wax synthase (WS) wherein said FAR and WS peptides operate to biosynthesize wax esters.
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A method of novel wax biosynthesis in algae comprising the steps of:
- transforming an algal cell with one or more polynucleotide sequences operably linked to a promoter that expresses:
- a heterologous fatty acyl-CoA reductase from Simmondsia chinensis according to amino acid sequence SEQ ID NO. 1, that reduces long-chain-fatty-acyl-CoA to acyl alcohol;
- a heterologous wax synthase from Simmondsia chinensis according to amino acid sequence SEQ ID NO. 2, that biosynthesizes wax esters from said acyl alcohol;
- culturing said algal cell;
- and
- harvesting the biosynthesized wax esters from the algal cell culture.
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A method of novel wax biosynthesis in algae comprising the steps of:
- transforming an algal cell with one or more polynucleotide sequences operably linked to a promoter that expresses:
- a heterologous fatty acyl-CoA reductase from Euglena gracilis according to amino acid sequence SEQ ID NO. 5, that reduces long-chain-fatty-acyl-CoA to acyl alcohol;
- and
- a heterologous wax synthase from Euglena gracilis according to amino acid sequence SEQ ID NO. 6, that biosynthesizes wax esters from said acyl alcohol;
- culturing said algal cell;
- and
- harvesting the biosynthesized wax esters from the algal cell culture.
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Dependencies are inferred from the claim text.