Compositions and methods for the biosynthesis of vanillin or vanillin beta-d-glucoside
AssigneesInternational Flavors & Fragrances Inc., Evolva SA
InventorsJoergen HANSEN, Esben Halkjaer HANSEN, Honey POLUR, Joseph M. SHERIDAN, Jonathan R. HEAL, William D.O. HAMILTON
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Abstract
Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express a mutant AROM polypeptide and/or mutant catechol-O-methyltransferase polypeptide alone or in combination with one or more vanillin biosynthetic enzymes or UDP-glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce vanillin or vanillin beta-D-glucoside.
Claims13
A method for producing vanillin and/or vanillin beta-D-glucoside comprising
- (a) providing a recombinant host capable of producing vanillin, wherein said recombinant host harbors a heterologous nucleic acid encoding Rhodococcus jostii, Rhodococcus opacus or Modestobacter marinus vanillyl alcohol oxidase;
- and
- (b) cultivating said recombinant host for a time sufficient for said recombinant host to produce vanillin and/or vanillin glucoside, wherein said recombinant host exhibits a reduction in the accumulation of vanillyl alcohol.
A recombinant host comprising a heterologous nucleic acid encoding Rhodococcus jostii, Rhodococcus opacus or Modestobacter marinas vanillyl alcohol oxidase.
Dependencies are inferred from the claim text.