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US 2021/0230641 A1Published application
Methods for recovering water-immiscible isoprenoid compounds from microbial biomass
AssigneeAmyris, Inc.
InventorsRonald Ray HENRY, Joshua Steven LENG
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Abstract
A method for recovering one or more water immiscible compounds comprising acidifying and disrupting the microbial biomass; heating the acidified, disrupted microbial biomass to form a heated, acidified disrupted microbial biomass; and contacting the heated, acidified, disrupted microbial biomass with a disulfonated surfactant in an amount sufficient to release at least 30% of the one or more water immiscible compounds from the microbial biomass.
Claims36
A method for recovering one or more water immiscible compounds from a microbial biomass, comprising the steps of:
- a. treating the microbial biomass by:
- i. acidifying the microbial biomass;
- and
- ii. disrupting the microbial biomass;
- to form an acidified, disrupted microbial biomass;
- b. heating the acidified, disrupted microbial biomass to form a heated, acidified, disrupted microbial biomass;
- c. contacting the heated, acidified, disrupted microbial biomass with a disulfonated surfactant in an amount sufficient to release at least 30% of the one or more water immiscible compounds from the microbial biomass;
- d. recovering the one or more water immiscible compounds.
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Dependencies are inferred from the claim text.