Method for producing homozygous cells
AssigneeOsaka University
InventorsYasuhide Yoshimura, Junji Takeda
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Abstract
The purpose of the present invention is to provide a technique by which a site-specific homozygote can be efficiently acquired by increasing cross efficiency and a homozygote in a genome modification-free state can be easily obtained. A method for producing homozygous cells, said method including: (A) a step for, into cells having a heterozygous mutation at a target site, introducing heterozygouse allele specific cleavage of DNA double strand of homologous chromosomes and thus inducing crossing in the presence of a Bloom's syndrome protein inhibitor to thereby give homozygous cells at the target site; and (B) a step for selecting the homozygous cells.
Claims13
A method for producing homozygous cells, comprising:
- (a) of causing crossover aiming at a target site on chromosome by introducing DNA double strand break that is specific to one allele of homologous chromosomes into cells having a heterozygous mutation at the target site in the presence of a Bloom's syndrome protein inhibitor to thereby obtain homozygous cells at the target site;
- and
- (b) selecting the homozygous cells.
Dependencies are inferred from the claim text.