Nucleic acid delivery carrier, nucleic acid delivery carrier set, nucleic acid delivery composition, and nucleic acid delivery method
AssigneeKabushiki Kaisha Toshiba
InventorsEiichi AKAHOSHI, Mitsuko ISHIHARA
Life of this patent
Estimating the term…
Abstract
According to one embodiment, a nucleic acid delivery carrier is used to integrate a first sequence into a genome of cells. The nucleic acid delivery carrier includes a donor DNA containing the first sequence, an RNA agent containing at least an RNA encoding a protein involving integration of the first sequence into the genome, and a lipid particle encapsulating the donor DNA and the RNA agent.
Claims30
A nucleic acid delivery carrier suitable for the integration of a DNA sequence into a genome of cells, the nucleic acid delivery carrier comprising:
- a donor DNA comprising the DNA sequence;
- an RNA agent comprising an RNA encoding a protein capable of integrating the DNA sequence into the genome of cells;
- and
- a lipid particle encapsulating the donor DNA and the RNA agent.
A nucleic acid delivery carrier set suitable for the integration of a DNA sequence into a genome of cells, the nucleic acid delivery carrier set comprising:
- a first carrier comprising a donor DNA comprising the DNA sequence and a first lipid particle encapsulating the donor DNA;
- and
- a second carrier comprising an RNA agent comprising an RNA encoding a protein capable of integrating the DNA sequence into the genome of cells, and a second lipid particle encapsulating the RNA agent.
A nucleic acid delivery method for integrating a DNA sequence into a genome of cells by using a nucleic acid delivery carrier, the method comprising:
- bringing the nucleic acid delivery carrier into contact with the cell,
- wherein the nucleic acid delivery carrier comprises
- a donor DNA comprising the DNA sequence,
- an RNA agent comprising an RNA encoding a protein capable of integrating the DNA sequence into the genome of cells, and
- a lipid particle encapsulating the donor DNA and the RNA.
A nucleic acid delivery method for integrating a DNA sequence into a genome of cells, the method comprising:
- bringing the first carrier and the second carrier into contact with the cell,
- wherein the first carrier comprises a donor DNA comprising the DNA sequence and a lipid particle encapsulating the donor DNA, and
- wherein the second carrier comprises an RNA agent comprising an RNA encoding a protein capable of integrating the DNA sequence into the genome of cells and a lipid particle encapsulating the RNA agent.
Dependencies are inferred from the claim text.