Methods and compositions related to improved nitrogen use efficiency
AssigneeAltria Client Services LLC
InventorsJesse FREDERICK, Andrew Carl ADAMS, Dongmei XU
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Abstract
The present disclosure provides products, compositions, and methods for improving nitrogen use efficiency in tobacco plants, including e.g., Burley tobacco. This disclosure further provides genetic markers for tracking enhanced nitrogen use efficiency phenotypes in tobacco plants and for introgressing enhanced nitrogen use efficiency phenotypes into tobacco plants. The disclosure also provides tobacco plants comprising enhanced nitrogen use efficiency and methods to the creation of tobacco plants comprising enhanced nitrogen use efficiency.
Claims20
A tobacco plant, or part thereof, comprising enhanced nitrogen use efficiency (NUE), wherein said tobacco plant comprises at least one functional allele of a Yellow Burley 1 (YB1) locus and further comprises at least one allele associated with enhanced NUE at one or more molecular markers selected from the group consisting of SEQ ID NOs:
- 57, 58, 59, 60, 61, 62, 63, and 64, wherein said enhanced NUE is relative to a control tobacco plant without said at least one functional allele of a YB1 locus.
A tobacco plant, or part thereof, comprising enhanced NUE, wherein said tobacco plant comprises at least one functional allele of a YB1 locus, and further comprises at least one allele associated with enhanced NUE at one or more molecular markers located within 10 cM of an allele associated with enhanced NUE at a sequence selected from the group consisting of SEQ ID NOs:
- 57, 58, 59, 60, 61, 62, 63, and 64.
A method of creating a tobacco plant or a population of tobacco plants comprising enhanced nitrogen use efficiency (NUE), said method comprising:
- a. providing a first population of tobacco plants comprising at least one enhanced NUE trait and second population of tobacco plants lacking said at least one enhanced NUE trait;
- b. genotyping said first population of tobacco plants for the presence of one or more molecular markers within 10 cM of an allele associated with enhanced NUE at a sequence selected from the group consisting of SEQ ID NOs:
- 57, 58, 59, 60, 61, 62, 63, and 64;
- c. selecting one or more tobacco plants of a first population of tobacco plants genotyped in step (b) that comprise said one or more molecular markers;
- d. crossing said at least one plant of said first population selected in step (c) with at least one plant of said second population that does not comprise said at least one enhanced NUE trait;
- and
- e. obtaining progeny plants or progeny seeds from step (d) that comprise said enhanced NUE trait, said allele associated with enhanced NUE, and at least one functional allele of a Yellow Burley 1 locus.
Dependencies are inferred from the claim text.