Plant regulatory elements and methods of use thereof
AssigneePioneer Hi-Bred International, Inc.
InventorsANDREW CARL CROW, SCOTT DIEHN, LYNNE EILEEN SIMS
Life of this patent
Estimating the term…
Abstract
The present disclosure relates to the field of plant molecular biology, more particularly to regulation of gene expression in plants.
Claims20
A recombinant polynucleotide comprising:
- (a) a polynucleotide having at least 95 percent sequence identity to the nucleic acid sequence of any one of SEQ ID NO:
- 1-12;
- (b) a polynucleotide of any one of SEQ ID NO:
- 1-12;
- or
- (c) a fragment of any one of SEQ ID NO:
- 1-12;
- wherein the recombinant polynucleotide has promoter activity.
A DNA construct comprising a heterologous transcribable polynucleotide molecule operably linked to the regulatory element polynucleotide, wherein the regulatory element polynucleotide comprises:
- (a) a polynucleotide having at least 95 percent sequence identity to the nucleic acid sequence of any one of SEQ ID NO:
- 1-12;
- (b) a polynucleotide of any one of SEQ ID NO:
- 1-12;
- or
- (c) a fragment of any one of SEQ ID NO:
- 1-12,
- wherein the regulatory element polynucleotide has promoter activity.
A method for expressing a polynucleotide in a plant comprising introducing into a plant cell a recombinant polynucleotide, said recombinant polynucleotide comprising a regulatory element capable of increasing expression of a heterologous polynucleotide, wherein said regulatory element comprises:
- (a) a nucleotide sequence of any one of SEQ ID NO:
- 1-12;
- (b) a sequence that is at least 95% identical to any one of SEQ ID NO:
- 1-12;
- or
- (c) a nucleotide sequence comprising a fragment or variant of the nucleotide sequence of any one of SEQ ID NO:
- 1-12, wherein the nucleotide sequence initiates transcription in a plant cell;
Dependencies are inferred from the claim text.