Plant regulatory elements and methods of use thereof
AssigneesPioneer Hi-Bred International, Inc., E. I. Du Pont de Nemours and Company
InventorsANDREW CARL CROW, SCOTT HENRY DIEHN, ALBERT LAURENCE LU, CARL ROBERT SIMMONS, LYNNE EILEEN SIMS
Life of this patent
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Abstract
The present disclosure relates to the field of plant molecular biology, more particularly to regulation of gene expression in plants.
Claims26
A recombinant polynucleotide selected from:
- (a) a polynucleotide having at least 95 percent sequence identity to the nucleic acid sequence of SEQ ID NO:
- 4, 5, 6, 7, 8, 9, 10, 11, 12, or 13;
- (b) a polynucleotide comprising the nucleic acid sequence of SEQ ID NOs:
- 4, 5, 6, 7, 8, 9, 10, 11, 12, or 13;
- (c) the polynucleotide of SEQ ID NO:
- 4, 5, 6, 7, 8, 9, 10, 11, 12, or 13;
- and
- (d) a fragment of SEQ ID NO:
- 4, 5, 6, 7, 8, 9, 10, 11, 12, or 13.
A DNA construct comprising a heterologous transcribable polynucleotide molecule operably linked to the regulatory element polynucleotide and a regulatory element polynucleotide selected from the group consisting of:
- (a) a polynucleotide having at least 95 percent sequence identity to the nucleic acid sequence of SEQ ID NO:
- 4, 5, 6, 7, 8, 9, 10, 11, 12, or 13;
- (b) a polynucleotide comprising the nucleic acid sequence of SEQ ID NO:
- 4, 5, 6, 7, 8, 9, 10, 11, 12, or 13;
- (c) the polynucleotide of SEQ ID NOs:
- 4, 5, 6, 7, 8, 9, 10, 11, 12, or 13;
- and
- (d) a fragment of SEQ ID NO:
- 4, 5, 6, 7, 8, 9, 10, 11, 12, or 13.
A recombinant polynucleotide comprising a heterologous enhancer polynucleotide sequence selected from:
- SEQ ID NO:
- 1, 2, or 3.
A method for expressing a polynucleotide in a plant, said method comprising introducing into a plant cell a recombinant polynucleotide, said recombinant polynucleotide comprising a regulatory element capable of increasing expression of a heterologous polynucleotide, wherein said regulatory element comprises a nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence comprising the nucleotide sequence of SEQ ID NO:
- 4, 5, 6, 7, 8, 9, 10, 11, 12, or 13;
- (b) a sequence that is at least 95% identical to SEQ ID NO:
- 4, 5, 6, 7, 8, 9, 10, 11, 12, or 13;
- (c) a nucleotide sequence comprising a fragment or variant of the nucleotide sequence of SEQ ID NOs:
- 4, 5, 6, 7, 8, 9, 10, 11, 12, or 13, wherein the sequence initiates transcription in a plant cell;
- and
- (d) a nucleotide sequence which is complementary to (a) or (b).
A method of enhancing transcription of a polynucleotide in a host cell comprising introducing into a plant cell a recombinant polynucleotide, said recombinant polynucleotide comprising a regulatory element capable of increasing expression of a heterologous polynucleotide of interest, wherein said regulatory element comprises a heterologous polynucleotide sequence selected from:
- SEQ ID NO:
- 1, 2, and 3.
Dependencies are inferred from the claim text.