Highly knotted molecular topologies from single-stranded nucleic acids
AssigneeArizona Board of Regents on behalf of Arizona State University
InventorsHao YAN, Fei ZHANG, Xiaodong QI
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Abstract
In some embodiments, complex molecular knots with high crossing numbers are achieved by folding, following a prescribed folding order, single-stranded DNA or RNA of customized sequences into target shapes. Such complex molecular knots with high crossing numbers are useful for biomedical applications including use as immunostimulatory agents and/or protein hosts and carriers.
Claims34
A knotted, self-assembled single-stranded nucleic acid (ssNA) nanostructure comprising a crossing number from 20 to 1,200 and comprising at least one paranemic cohesion crossover,
- wherein the nucleic acid is selected from DNA or RNA,
- wherein the NA sequences is from, about 1800 to about 7500 nucleotides in length, and
- wherein the nanostructure self-assembles by means of paranemic cohesion into knots comprising four ssNA regions.
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