Kit, system, and flow cell

A kit, comprising:

• an RNA to cDNA conversion sub-kit;
• and
• an enrichment fluid including target capture beads, each target capture bead including:
• a solid support;
• and
• capture probes attached to the solid support, each of the capture probes including a single stranded deoxyribonucleic acid sequence that is complementary to a targeted region of cDNA generated using the RNA to cDNA conversion sub-kit.

A system, comprising:

• a substrate;
• a preparation channel defined at a first region on the substrate, the preparation channel including preparation capture sites to immobilize library preparation beads;
• an enrichment channel defined at a second region on the substrate downstream of the preparation channel, the enrichment channel including:
• amplification primers;
• and
• enrichment capture sites to immobilize target capture beads;
• and
• a transport channel selectively and fluidically connecting the preparation channel and the enrichment channel.

A flow cell, comprising:

• an enrichment channel defined between two surfaces, the enrichment channel, including:
• amplification primers attached to at least one of the two surfaces;
• and
• target capture beads immobilized on the at least one of the two surfaces, each target capture bead including:
• a solid support;
• and
• capture probes attached to the solid support, each of the capture probes including a single stranded nucleic acid sequence that is complementary to a targeted region of a genomic deoxyribonucleic acid or of a complementary deoxyribonucleic acid.

A method, comprising:

• introducing target capture beads into an enrichment channel including at least one surface containing amplification primers and enrichment capture sites, whereby at least some of the target capture beads become immobilized at the enrichment capture sites, each of the target capture beads including:
• a solid support;
• and
• capture probes attached to the solid support, each of the capture probes including a single stranded nucleic acid sequence that is complementary to a targeted region of a genomic deoxyribonucleic acid or of a complementary deoxyribonucleic acid.