High Fidelity Restriction Endonucleases
AssigneeNew England Biolabs, Inc.
InventorsZhenyu Zhu, Aine Quimby, Shuang-Yong Xu, Shengxi Guan, Hua Wei, Penghua Zhang, Dapeng Sun, Siu-hong Chan
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Abstract
Compositions and methods are provided for enzymes with altered properties that involve a systematic approach to mutagenesis and a screening assay that permits selection of the desired proteins. Embodiments of the method are particularly suited for modifying specific properties of restriction endonucleases such as star activity. The compositions includes restriction endonucleases with reduced star activity as defined by an overall fidelity index improvement factor.
Claims10
1-43. (canceled)
A composition comprising a variant BspQI restriction endonuclease, wherein the variant BspQI restriction endonuclease comprises an amino acid sequence that differs from the amino acid sequence of the parent BspQI restriction endonuclease by one or more amino acids substitutions.
Dependencies are inferred from the claim text.