Back to results
US 2021/0230580 A1Published application
Quality control reagents and methods for serum antibody profiling
AssigneeSerimmune Inc.
InventorsKathryn Vinaya Louise Kamath, Jack Ryan Reifert, Patrick Sean Daugherty
Life of this patent
Estimating the term…
Abstract
Disclosed herein are reagents for use in quality control assessment of antibody profiling platforms, such as biopanning and Digital Serology. Also disclosed herein are kits, method of manufacturing, and methods of using the same.
Claims108
A peptide expression library composition comprising:
- a) a library of nucleic acid sequences encoding a library of peptides;
- b) one or more control vectors comprising:
- 1) a nucleic acid sequence encoding a control binding target of a control binding molecule, wherein the control binding target is identical for each of the control vectors, and
- 2) a unique nucleic acid sequence, wherein the unique nucleic acid sequence is specific for each of the control vectors.
1 more element
A peptide library composition comprising:
- a) a library of nucleic acid sequences encoding a library of random peptides;
- b) two control vectors, each comprising:
- 1) a nucleic acid sequence encoding an epitope sequence that is a known binding target of a control binding molecule, wherein the control binding molecule comprises an antibody or fragment thereof, wherein the epitope sequence is identical for each of the control vectors, and
- 2) a unique nucleic acid sequence, wherein the unique nucleic acid sequence is specific for each of the control vectors, and
- c) two or more array surfaces,
- wherein the library of random peptides and the epitope sequence are capable of being expressed, and wherein each of the array surfaces is configured to display multiple copies of:
- 1) one of the random peptides, or
- 2) the epitope sequence encoded by one of the control vectors.
5 more elements
A kit comprising the composition of any of the above claims and instructions for use.
A method of manufacturing any of the compositions or kits of the above claims.
Dependencies are inferred from the claim text.