Use of nanosecond pulsed electric field in improving cell stemness
AssigneePeking University
InventorsZigang GE, Tong NING, Jue ZHANG, Jiaqing CHEN, Jinsong GUO, Kejia LI
Life of this patent
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Abstract
Provided is the use of a nanosecond pulsed electric field in improving cell stemness. Specifically, applying a nanosecond pulsed electric field to cells, especially stem cells, can effectively increase the expression level of stemness genes and reduce the methylation level of the cells, improve the stemness of the stem cells, and further facilitate the ability of the stem cells to receive induced differentiation.
Claims16
1-5. (canceled)
A method for enhancing stemness, increasing the expression of stemness-related genes, reducing the expression of methylation genes and/or reducing the methylation level in a cell, comprising applying an electric field to the cell to give an electric shock to the cell, wherein, the electric field is a nanosecond pulsed electric field, and the cell is a stem cell.
10-11. (canceled)
The method according to claim 19, wherein the conductive film is prepared by blend-casting, comprising:
- 1) dissolving the poly-L-lactic acid in a solvent until fully dissolved;
- 2) dispersing the multi-walled carbon nanotubes in the same solvent;
- 3) performing ultrasonication on the multi-walled carbon nanotubes mixture of step 2), and adding into the poly-L-lactic acid solution of step 1), to allow the final concentration of poly-L-lactic acid is 1-50% by w/v;
- 4) after ultrasonication, casting the resultant on a plate and standing;
- 5) peeling off the film formed on the plate;
- and
- 6) placing the film into an oven to fully evaporate the solvent, and obtain a conductive film.
Dependencies are inferred from the claim text.