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AssigneeQingdao Kingagroot Chemical Compound Co., Ltd.
InventorsLei LIAN, Sudong MO, Huarong LI, Guangdi YUAN, Zhenguo LI, Junjie ZHANG, Dehui DING, Bo CHEN, Guizhi LIU, Chao SONG, Lei WANG
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The present invention relates to a mutant p-hydroxyphenylpyruvate dioxygenase (HPPD) protein or a bioactive fragment thereof and an isolated polynucleotide comprising a nucleic acid sequence encoding the protein or fragment thereof, wherein the mutant p-hydroxyphenylpyruvate dioxygenase (HPPD) protein or a bioactive fragment thereof retains or enhances the property of catalyzing the conversion of p-hydroxyphenylpyruvate (HPP) to homogentisate and is significantly less sensitive to HPPD-inhibiting herbicides than a wild-type HPPD. The present invention also relates to a nucleic acid construct, an expression vector and a host cell comprising the polynucleotide, as well as to a method for producing a plant that has the property of catalyzing the conversion of p-hydroxyphenylpyruvate (HPP) to homogentisate and significantly reduced sensitivity to HPPD-inhibiting herbicides.
A mutant p-hydroxyphenylpyruvate dioxygenase (HPPD) protein or a bioactive fragment thereof, wherein, as compared to the amino acid sequence of a wild-type rice p-hydroxyphenylpyruvate dioxygenase protein, the amino acid sequence of the mutant p-hydroxyphenylpyruvate dioxygenase (HPPD) protein has one or more of mutations selected from the group consisting of 93S, 103S, 141R, 141K, 141T, 165V, 191I, 220K, 226H, 276W, 277N, 336D, 337A, 338D, 338S, 338Y, 342D, 346C, 346D, 346H, 346S, 346Y, 370N, 377C, 386T, 390I, 392L, 403G, 410I, 418P, 419F, 419L, 419V, 420S, 420T, 430G, and 431L at one or more positions corresponding to 93, 103, 141, 165, 191, 220, 226, 276, 277, 336, 337, 338, 342, 346, 370, 377, 386, 390, 392, 403, 410, 418, 419, 420, 430 and 431 of the amino acid sequence of wild-type rice p-hydroxyphenylpyruvate dioxygenase protein as set forth in SEQ ID NO:
A method for producing a mutant p-hydroxyphenylpyruvate dioxygenase (HPPD) protein which retains or enhances the property of catalyzing the conversion of p-hydroxyphenylpyruvate (HPP) to homogentisate and is significantly less sensitive to HPPD-inhibiting herbicides than a wild-type HPPD, comprising the steps of:
Dependencies are inferred from the claim text.