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AssigneeInstitute of Animal Science, Chinese Academy of Agricultural Sciences
InventorsBin YAO, Huiying LUO, Tao TU, Huoqing HUANG, Xiaoyun SU, Yaru WANG, Yingguo BAI, Yuan WANG, Kun MENG
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The present invention takes the glucose oxidase GOD from Aspergillus niger as the mutation template to obtain the glucose oxidase GOD mutants with improved catalytic efficiency and thermal stability by site directed mutagenesis. The specific activity of the mutant of the present invention is 66% higher than that of the wild type GOD; the enzyme activity of the mutant of the present invention is 13.6 times higher than that of the wild type after being treated at 70° C. for 10 min; and the enzyme activity of the mutant of the present invention is 29.4 times higher than that of the wild type after being treated at 80° C. for 2 min.
The glucose oxidase GOD mutant which is obtained by muting the amino acid at the site of 82 of the glucose oxidase GOD having the amino acid sequence of SEQ ID No:
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