Synthetic chimeric vaccinia virus
AssigneeTonix Pharma Limited
InventorsDavid Evans, Ryan Noyce, Seth Lederman
Life of this patent
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Abstract
The invention relates in various aspects to a synthetic chimeric vaccinia virus or compositions comprising such viruses, and the development and use of systems and methods for producing such synthetic chimeric vaccinia viruses. The synthetic chimeric vaccinia viruses are well suited, among others, as virus vaccines or to generate an oncolytic response and pharmaceutical formulations.
Claims55
A synthetic chimeric vaccinia virus (scVACV) that is replicated and reactivated from DNA derived from synthetic DNA, the viral genome of said virus differing from a wild type genome of said virus in that it is characterized by one or more modifications.
A method of producing a synthetic chimeric vaccinia virus (scVACV) comprising the steps of:
- (i) chemically synthesizing overlapping DNA fragments that correspond to substantially all of the viral genome of the vaccinia virus;
- (ii) transfecting the overlapping DNA fragments into helper virus-infected cells;
- (iii) culturing said cells to produce a mixture of helper virus and synthetic chimeric vaccinia virus particles in said cells;
- and
- (iv) plating the mixture on host cells specific to the scVACV to recover the scVACV.
Dependencies are inferred from the claim text.