Propagator cells and methods for propagating phage, in particular for delivering crispr-cas components via probiotic organisms
AssigneeSNIPR Technologies Limited
InventorJasper CLUBE
Life of this patent
Estimating the term…
Abstract
The invention provides propagator cells and methods for propagating phage and transduction particles.
Claims27
A method of producing a population of phages, wherein the phages are of a first type capable of infecting host cells of a first bacterial species or strain by binding a cell-surface receptor comprised by bacteria of said first species or strain, the method comprising
- (a) Providing a population of second bacterial cells comprising the receptor on the surface of the second cells, wherein the second cells are of a second species or strain, wherein the second species or strain is different from the first species or strain;
- (b) Infecting the second cells with phages of said first type;
- and
- (c) Propagating the phages in the second cells, thereby producing the population of phages.
A method of producing a population of transduction particles comprising nucleic acid packaged by phage coat proteins, wherein the particles are capable of infecting host cells of a first bacterial species or strain by binding a cell-surface receptor comprised by bacteria of said first species or strain, whereby host cells are transduced with the nucleic acid, the method comprising
- (a) Providing a population of second bacterial cells comprising the receptor on the surface of the second cells, wherein the second cells are of a second species or strain, wherein the second species or strain is different from the first species or strain, and wherein the second cells comprise DNA that is capable of producing copies of said nucleic acid;
- (b) Infecting the second cells with phage encoding the phage coat proteins by binding the phage to the receptor comprised by the second bacterial cells;
- and
- (c) Propagating the phage in the second cells, wherein phage coat proteins are produced that package copies of said nucleic acid, thereby producing the population of particles.
(canceled)
(canceled)
A propagator cell for propagating phage or transduction particles comprising nucleic acid packaged by phage coat proteins, wherein the phage or particles are of a first type capable of infecting host cells of a first bacterial species or strain by binding a cell-surface receptor comprised by bacteria of said first species or strain, the propagator cell comprising the receptor on the surface thereof, wherein the propagator cell is of a second species or strain, wherein the second species or strain is different from the first species or strain, whereby the propagator cell is capable of being infected by phage of said first type or said particles for propagation of phage or particles respectively therein.
23-34. (canceled)
36-37. (cancelled)
Dependencies are inferred from the claim text.