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US 2021/0230554 A1Published application
Generation of functional beta cells from human pluripotent stem cell-derived endocrine progenitors
OwnerNicolaj Stroeyer Christophersen, Ulrik Doehn +1inventors
InventorsNicolaj Stroeyer Christophersen, Ulrik Doehn, Mattias Hansson
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Abstract
The present invention relates to method of generation of functional mature beta cells from human pluripotent stem cell-derived endocrine progenitors. The present invention also relates to functional mature beta cells produced by said methods and uses of said mature beta cells for treating diabetes.
Claims16
A method for generating functional mature beta cells from endocrine progenitor cells, comprising:
- (1) culturing the endocrine progenitor cells in a cell culture medium comprising a first serum replacement medium, histone methyltransferase EZH2 inhibitor, TGF-beta signaling pathway inhibitor, and Heparin, to obtain INS+ and NKX6.1+ double positive immature beta cells and
- (2) culturing the INS+ and NKX6.1+ double positive immature beta cells of step (1) in a cell culture medium comprising a second serum replacement medium, to obtain the functional mature beta cells.
(canceled)
Dependencies are inferred from the claim text.