Cell induction method

Abstract

The present invention relates to a cell induction method, in particular to a method for inducing differentiation of fibroblasts into adipocytes, comprising the following steps: Cultivating the fibroblasts in a culture medium, adding a Myosin inhibitor and BMP4 to the culture medium, and continuously cultivating same until adipocytes are obtained. The present invention further relates to a method for inducing the conversion of fibroblasts into immortalized cells and use thereof. The method comprises the following steps: cultivating the fibroblasts in a culture medium, adding Myosin inhibitor to the culture medium, and continuously cultivating same until immortalized cells are obtained. The present invention further relates to a method for inducing in vitro amplification of hepatocytes. The present invention further relates to use of a Myosin inhibitor, especially (-)-Blebbistatin or (S)-(-)-Blebbistatin, O-Benzoate, and hepatocytes obtained by means of the method for inducing in vitro amplification of hepatocytes, constructing of bioartificial livers, and constructing models of liver disease.

Dependencies are inferred from the claim text.