Methods for isolation of platelets
AssigneeCelularity Inc.
InventorsManoochehr KHORSHIDI, Robert J. HARIRI, Xiaokui ZHANG
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Abstract
Provided herein are methods for the isolation of platelets, for example, isolation of platelets from umbilical cord blood. In certain embodiments, presented herein are methods for preparation of platelet rich plasma. In one aspect, provided herein are methods for isolation of platelets from blood. In certain embodiments, presented herein are methods for isolation of platelets from cord blood, e.g., human cord blood. The isolated platelets can be used for a variety of applications, including, for example, methods of wound healing, organ repair and/or regeneration, and/or tissue repair and/or regeneration, in either autologous or allogeneic settings.
Claims34
A method for isolation of platelets from umbilical cord blood or blood obtained from placenta, the method comprising:
- removing erythrocytes from the umbilical cord blood or blood obtained from placenta to produce plasma, and separating leukocytes from the plasma.
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A method for isolation of platelets from umbilical cord blood or blood obtained from a placenta, the method comprising:
- centrifuging the blood at about 100×G to about 500×G for about 10 minutes to about 30 minutes, thereby producing PRP.
The method of 20, further comprising storing the freeze dried PRP at room temperature under vacuum.
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The method of 28, further comprising storing the freeze dried platelets at room temperature under vacuum.
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Dependencies are inferred from the claim text.