Kits, compositions and methods for cell enrichment
AssigneeStemcell Technologies Canada Inc.
InventorFrann Antignano
Life of this patent
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Abstract
Kits, compositions and methods for enriching myeloid-derived suppressor cells (MDSCs) are provided. In one embodiment, the kit comprises (a) one or more antibodies or fragments thereof that bind one or more non-MDSC target cells; and (b) a plurality of particles, wherein the plurality of particles are linked or linkable to at least one of the one or more antibodies.
Claims27
A kit for enriching myeloid-derived suppressor cells (MDSCs) from a sample comprising target cells and MDSCs, the kit comprising:
- one or more antibodies or fragments thereof that bind one or more target cells;
- and a plurality of particles, wherein the plurality of particles are linked or linkable to at least one of the one or more antibodies or fragments thereof.
The kit according to any one of claim[[s 1-]] 3, wherein the target cells comprise:
- (a) T cells, B cells, red blood cells, granulocytes, dendritic cells, and NK cells,
- (b) T cells, B cells, red blood cells, granulocytes, dendritic cells, NK cells and macrophages,
- (c) T cells, B cells, red blood cells, granulocytes, dendritic cells, NK cells, macrophages, mast cells and hematopoietic progenitors,
- (d) T cells, B cells, red blood cells, granulocytes, dendritic cells, NK cells, mast cells and hematopoietic progenitors, or
- (e) T cells, B cells, red blood cells, dendritic cells, NK cells, macrophages, mast cells, hematopoietic progenitors, monocytes, granulocytes and endothelial cells.
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A method for enriching myeloid-derived suppressor cells (MDSCs) from a sample comprising target cells and MDSCs, the method comprising:
- contacting the sample with one or more antibodies or fragments thereof that bind one or more target cells, and a plurality of particles, wherein the plurality of particles are linked or linkable to at least one of the antibodies;
- incubating the target cells, antibodies, and particles to form target cell:particle complexes;
- and
- separating the target cell:particle complexes from the sample to enrich the MDSCs.
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Dependencies are inferred from the claim text.