Umbilical cord lining stem cells and conditioned media thereof
OwnerRafael Gonzalezinventor
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Abstract
The present disclosure relates to compositions of ULSCs and/or conditioned media thereof. In particular, the disclosure relates to conditioned media containing therapeutic factors including growth factors, cytokines, chemokines and extra cellular matrix components.
Claims3
A composition of umbilical cord lining stem cells (ULSCs) and a conditioned media thereof comprising a Stem Cell Factor (SCF), a Vascular Endothelial Growth Factor (VEGF), a Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), an Interleukin (IL) 4, 7, 8, a Tumor Necrosis Factor alpha (TNF-α), a Macrophage Inflammatory Protein (MIP) 1β, and a Monocyte Chemoattractant Protein (MCP) 1, Chondroitin Sulfate, Hyaluronic Acid and Collagen.
A concentrated composition of ULSCs conditioned media comprising:
- a. a SCF from 20 to 50 picograms per milliliter (pg/ml);
- and
- b. a VEGF from 200 to 1200 pg/ml;
- and
- c. a cytokine selected from the group consisting of a GM-CSF, an IL4, 7, 8, a TNF-α at levels up to 4 times greater than in bone marrow mesenchymal stem cells (BMSCs) conditioned media;
- and
- d. a chemokine comprising a MIP-1β, and a MCP-1 at levels up to 4 times greater than in BMSCs conditioned media;
- and
- e. an extracellular matrix (ECM) component comprising a collagen I, a collagen III, a chondroitin sulfate (CS), and a hyaluronic acid (HA) at levels comparable to or greater than in BMSCs conditioned media;
- and
- f. said HA being of high molecular weight up to at least 2500 kilodaltons (kd);
- and
- g. said collagen I and said collagen III from 0.75 to 1 milligrams per milliliter (mg/ml).
A concentrated and sterilized composition of ULSCs derived conditioned media which comprises:
- a. a SCF from 20 to 50 pg/ml;
- and
- b. a VEGF from 200 to 1200 pg/ml;
- and
- c. a cytokine selected from the group consisting of a GM-CSF, an IL4, 7, 8, a TNF-α at levels up to 4 times greater than in BMSCs conditioned media;
- and
- d. a chemokine comprising a MIP-1β, and a MCP-1 at levels up to 4 times greater than in BMSCs conditioned media;
- and
- e. an extracellular matrix (ECM) component comprising a collagen I, a collagen III, a chondroitin sulfate (CS), and a hyaluronic acid (HA) at levels comparable to or greater than in BMSCs conditioned media;
- and
- f. said HA being of high molecular weight up to at least 2500 kilodaltons (kd);
- g. said collagen I and said collagen III from 0.75 to 1 milligrams per milliliter (mg/ml).
Dependencies are inferred from the claim text.