Method for improving affinity of antibody for antigen and use thereof
AssigneeSysmex Corporation
InventorsShingo MAETA, Atsushi FUKUNAGA, Reema BAJAJ
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Abstract
Disclosed is a method for improving affinity of an antibody for an antigen, comprising, in an unmodified antibody, improving affinity for an antigen as compared to the unmodified antibody, by changing 17th, 18th and 20th amino acid residues of a light chain defined by Kabat method to charged amino acid residues.
Claims15
A method for improving affinity of an antibody for an antigen, comprising, in an unmodified antibody, improving affinity for an antigen as compared to the unmodified antibody, by changing 17th, 18th and 20th amino acid residues of a light chain defined by Kabat method to charged amino acid residues.
A method for producing an antibody with improved affinity for an antigen as compared to an unmodified antibody, comprising:
- in an unmodified antibody, changing 17th, 18th and 20th amino acid residues of a light chain defined by Kabat method to charged amino acid residues;
- and
- recovering the antibody obtained in the changing.
A modified antibody with improved affinity for an antigen as compared to an unmodified antibody, wherein 17th, 18th and 20th amino acid residues of a light chain defined by Kabat method in the unmodified antibody are changed to charged amino acid residues.
Dependencies are inferred from the claim text.