Antibody targeting cldn18.2, bispecific antibody, adc, and car, and applications thereof

An antibody targeting CLDN18.2, wherein the antibody targeting CLDN18.2 comprises a light chain variable region (VL) or a heavy chain variable region (VH), wherein the VL comprises the following complementary determining region (CDR) sequences:

• a VL CDR1 amino acid sequence as shown in SEQ ID NO:
• 11 or SEQ ID NO:
• 12;
• a VL CDR2 amino acid sequence as shown in SEQ ID NO:
• 13;
• and a VL CDR3 amino acid sequence as shown in SEQ ID NO:
• 14;
• and the VH comprises the following CDR sequences:
• a VH CDR1 amino acid sequence as shown in SEQ ID NO:
• 15;
• a VH CDR2 amino acid sequence as shown in SEQ ID NO:
• 16;
• and a VH CDR3 amino acid sequence as shown in SEQ ID NO:
• 17;
• preferably, the VL of the antibody targeting CLDN18.2 comprises amino acid sequences of VL CDR1 as shown in SEQ ID NO:
• 11, VL CDR2 as shown in SEQ ID NO:
• 13 and VL CDR3 as shown in SEQ ID NO:
• 14:
• the VH of the antibody targeting CLDN18.2 comprises amino acid sequences of VH CDR1 as shown in SEQ ID NO:
• 15, VH CDR2 as shown in SEQ ID NO:
• 16 and VH CDR3 as shown in SEQ ID NO:
• 17;
• or,
• the VL of the antibody targeting CLDN18.2 comprises amino acid sequences of VL CDR1 as shown in SEQ ID NO:
• 12, VL CDR2 as shown in SEQ ID NO:
• 13 and VL CDR3 as shown in SEQ ID NO:
• 14;
• and the VH of the antibody targeting CLDN18.2 comprises amino acid sequences of VH CDR1 as shown in SEQ ID NO:
• 15, VH CDR2 as shown in SEQ ID NO:
• 16 and VH CDR3 as shown in SEQ ID NO:
• 17:
• or, the CDR mutation sequence of the antibody targeting CLDN18.2 is a sequence with a deamination sensitive site mutation in a CDR region;
• more preferably, the deamination sensitive site in the CDR region is position L30A or L30B of light chain CDR1;
• or, position H99 or H100 of heavy chain CDR3;
• even more preferably, the amino acid residues at positions L30A and L30B of light chain CDR1 are mutated from NS to TS or NT, provided that position L30E is not Q and position L34 is not T;
• the amino acid residues at positions H99 and H100 of heavy chain CDR3 are mutated from NS to TS or NT, provided that in light chain CDR1, position L30E is not 0 and position L34 is not T.