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US 2021/0230232 A1Published application
Site-specific bio-conjugation methods and compositions useful for nanopore systems
AssigneeRoche Sequencing Solutions, Inc.
InventorsDmitriy GREMYACHINSKIY, Christos TZITZILONIS
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Abstract
The present disclosure relates to relates methods and associated compositions that provide fast, efficient site-selective conjugation of a protein, such as the pore-forming protein α-hemolysin, to a biomolecule, such as a DNA polymerase, and the use of such site-selective protein-biomolecule conjugates in nanopore devices and methods.
Claims13
A composition comprising a modified pore-forming protein of structural formula (IVa)
- wherein,
- S is a sulfur atom of a thiol group of the pore forming protein;
- A is a thiol reactive group;
- LA is a linker;
- and
- X is a click chemistry reactive group;
- Y is a click chemistry reactive group that undergoes a click chemistry reaction with the reactive group X;
- LB is a linker;
- and
- B is a reactive group.
7 more elements
Dependencies are inferred from the claim text.