Hcmv vaccine strain
AssigneeBodo PLACHTER
InventorsBodo PLACHTER, Christine ZIMMERMANN
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Abstract
The present invention relates to nucleic acid molecules encoding a recombinant human cytomegalovirus (HCMV) strain, dense bodies produced by said HCMV strain and preparations of said dense bodies for use in medicine, particularly as a vaccine against HCMV.
Claims29
A nucleic acid molecule encoding the genome of a recombinant HCMV strain,
- wherein the recombinant HCMV strain does not encode a functional pUL25 protein, and wherein the recombinant HCMV strain does not encode functional interferon-ß, for use in medicine, particularly in human medicine.
A method of producing an HCMV dense body, comprising the steps:
- (a) infecting a mammalian target cell with an HCMV strain comprising at least one gene encoding a replication-essential HCMV protein fused to a destabilizing protein domain under conditions wherein a stabilizing ligand of said destabilizing protein domain is present, and
- (b) culturing the cell under conditions wherein the stabilizing ligand is absent, and an HCMV dense body is produced.
A HCMV dense body produced by infection of a mammalian target cell with a recombinant HCMV strain encoded by a nucleic acid molecule comprising at least one replication-essential HCMV gene fused to a gene encoding a destabilizing protein domain.
Use of a recombinant HCMV strain encoded by a nucleic acid molecule comprising at least one replication-essential HCMV gene fused to a gene encoding a destabilizing protein domain for the production of a HCMV dense body-preparation.
Use of an HCMV particle produced by infection of a mammalian target cell with a recombinant HCMV strain encoded by a nucleic acid molecule comprising at least one replication-essential HCMV gene fused to a gene encoding a destabilizing protein domain for the production of a HCMV dense body-preparation.
Use of a recombinant HCMV strain encoded by a nucleic acid molecule comprising at least one replication-essential HCMV gene fused to a gene encoding a destabilizing protein domain for increasing the safety of a HCMV vaccine.
Use of an HCMV particle produced by infection of a mammalian target cell with a recombinant HCMV strain encoded by a nucleic acid molecule comprising at least one replication-essential HCMV gene fused to a gene encoding a destabilizing protein domain for increasing the safety of a HCMV vaccine.
Dependencies are inferred from the claim text.