SIMPLE METHOD FOR THE PURIFICATION OF LACTO-N-NEOTETRAOSE (LNnT) FROM CARBOHYDRATES OBTAINED BY MICROBIAL FERMENTATION
AssigneeJennewein Biotechnologie GmbH
InventorsStefan JENNEWEIN, Markus HELFRICH
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Abstract
Provided is a method for the purification of lacto-N-neotetraose from other carbohydrates, characterized in that the method comprises the steps of subjecting an aqueous solution containing lacto-N-neotetraose to two membrane filtration steps using different membranes or of subjecting an aqueous solution containing lacto-N-neotetraose to a membrane filtration step and a continuous chromatography.
Claims18
A method for purification of lacto-N-neotetraose from other carbohydrates, wherein the method comprises subjecting an aqueous solution comprising lacto-N-neotetraose to two membrane filtrations using different membranes, wherein
- one membrane has a molecular weight cut-off of between about 300 to about 500 Dalton, and
- the other membrane as a molecular weight cut-off of between about 600 to about 800 Dalton.
A method for purification of lacto-N-neotetraose from other carbohydrates, wherein the method comprises subjecting an aqueous solution containing lacto-N-neotetraose to membrane filtration using a membrane having a molecular weight cut-off of between about 300 to about 500 Dalton, and subjecting the aqueous solution to a continuous chromatography to remove carbohydrates having a molecular weight larger than that of LNnT from the aqueous solution.
A method for purification of lacto-N-neotetraose from other carbohydrates, wherein the method comprises subjecting an aqueous solution comprising lacto-N-neotetraose to membrane filtration using a membrane having a molecular weight cut-off of between about 600 to about 800 Dalton, and subjecting the aqueous solution to a continuous chromatography to remove carbohydrates having a molecular weight smaller than that of LNnT from the aqueous solution.
Dependencies are inferred from the claim text.