Digital pcr chip, and droplet generation system and detection system containing same

Abstract

Disclosed are a digital PCR chip, and a droplet generation system and detection system containing same. The digital PCR chip comprises a chip body having a droplet storage cavity, and a liquid inlet provided in the chip body; and the digital PCR chip further comprises a containing cavity, which stands on the chip body and is in communication with the liquid inlet, and a liquid outlet provided in the chip body. The chip body further comprises a first channel enabling the liquid inlet to be in communication with the droplet storage cavity, and a second channel enabling the liquid outlet to be in communication with the droplet storage cavity, wherein the first channel has a first internal channel located inside the chip body, and the second channel has a second internal channel located inside the chip body. Droplets are generated in the containing cavity, and then pass through the liquid inlet and the first channel to enter the droplet storage cavity. The droplets can maintain good stability and a good sealing performance during conveyance, and the droplets are uniformly laid flat in a single layer or in multiple layers in the droplet storage cavity, thereby facilitating obtaining a markedly more accurate detection result.

Dependencies are inferred from the claim text.