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US 2021/0229095 A1Published application
Integrated platforms for precise poly-sensing and imaging
AssigneePolybiomics, Inc.
InventorsMandana Veiseh, Seyed Bahram Bahrami, Michial Duff Howell, James Ferguson
Life of this patent
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Abstract
A hybrid biosensing-imaging system that can operate inside an incubator, structures and methods are directed to placing modular and removable biosensors and biocompatible interfaces in 3D transparent test wells that contain biological samples. The technology enables continuous monitoring of multiple simultaneous parameters and functions of a living cell or cell clusters such as alterations of cellular ligands, physicochemical biomarkers, phenotypes, and/or extracellular compositions upon interactions with analytes or during progressions. Methods of capturing and analyzing direct orthogonal information from biological samples in 2D and 3D.
Claims53
A device comprising:
- a. a test plate comprising an electrical interconnects substrate and a well slide coupled to the electrical interconnects substrate, where the well slide includes at least one well, each well configured to contain a substance to be analyzed, wherein each well comprises a side wall structure and a bottom wall;
- b. a plurality of sensors configured to sense a plurality of different modalities, wherein each sensor is configured to sense a characteristic of the substance according to one of the modalities and to generate a corresponding sensed signal, further wherein at least one of the plurality of sensors is attached to the bottom wall or the side wall structure of each well;
- c. one or more probes inserted into each well, each probe includes an optical path, wherein the plurality of sensors includes at least one chemical sensor configured to sense according to a chemical measuring modality, further wherein each probe includes the chemical sensor coupled to an end of the optical path;
- and
- d. a measurement device coupled to the optical path.
2 more elements
A device comprising:
- a. a test plate comprising an electrical interconnects substrate and a well slide coupled to the electrical interconnects substrate, where the well slide includes at least one well, each well configured to contain a substance to be analyzed, wherein each well comprises a bottom wall and a transparent side wall structure;
- b. a plurality of sensors configured to sense a plurality of different modalities, wherein each sensor is configured to sense a characteristic of the substance according to one of the modalities and to generate a corresponding sensed signal, further wherein at least one of the plurality of sensors is attached to the bottom wall or the side wall structure of each well;
- c. one or more optical paths external to the well slide and aligned with the side wall structure of each well, wherein the plurality of sensors includes at least one chemical sensor configured to sense according to a chemical measuring modality, the at least one chemical sensor is positioned on an interior surface of the side wall structure of each well;
- and
- d. a measurement device coupled to the optical path.
2 more elements
A device comprising:
- a. a test plate comprising an electrical interconnects substrate and a well slide coupled to the electrical interconnects substrate, where the well slide includes at least one well, each well configured to contain a substance to be analyzed, wherein each well comprises a side wall structure and a transparent bottom wall, further wherein the electrical interconnects substrate comprises a transparent material;
- b. a plurality of sensors configured to sense a plurality of different modalities, wherein each sensor is configured to sense a characteristic of the substance according to one of the modalities and to generate a corresponding sensed signal, further wherein at least one of the plurality of sensors is a chemical sensor attached to the bottom wall or the side wall structure of each well, the chemical sensor configured to sense according to a chemical measuring modality;
- and
- c. an imaging unit coupled to the test plate, wherein the imaging unit comprises a light source and illumination optics to direct light into each well, the light source is configured to selectively generate different light wavelengths, further wherein the imaging unit further comprises camera with a sensing element coupled to receive light from each of the wells.
1 more element
A device comprising:
- a. a test plate comprising an electrical interconnects substrate and a well slide coupled to the electrical interconnects substrate, wherein the well slide includes a plurality of wells, each well configured to contain a same substance to be analyzed, wherein each well comprises a side wall structure and a bottom wall;
- and
- b. a plurality of sensors configured to sense a plurality of different modalities, wherein each sensor is configured to sense a characteristic of the substance according to one of the modalities and to generate a corresponding sensed signal, further wherein each well includes one or more senors of a single modality, each well has sensors of a different modality from each of the other wells.
A device comprising:
- a. a test plate comprising an electrical interconnects substrate and a well slide coupled to the electrical interconnects substrate, where the well slide includes at least one well, each well configured to contain a substance to be analyzed, wherein each well comprises a side wall structure and a bottom wall;
- b. one or more inserts each comprising a substrate having conductive interconnects and one or more of the plurality of sensors, wherein each insert is positioned in a corresponding well;
- and
- c. a plurality of sensors configured to sense a plurality of different modalities, wherein each sensor is configured to sense a characteristic of the substance according to one of the modalities and to generate a corresponding sensed signal, further wherein at least one of the plurality of sensors is attached to the bottom wall or the side wall structure of each well, and at least one of the plurality of sensors is attached to each insert, wherein the plurality of sensors enable 2D and 3D polysensing of the substance within each well.
1 more element
Dependencies are inferred from the claim text.