Separation medium, use for separation medium, steviol glycoside separation method using separation medium, and steviol glycoside production method using separation method
AssigneeMitsubishi Chemical Corporation
InventorTadashi ADACHI
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Abstract
An object of the present invention is to provide a separation medium and a separation method, ensuring high selectivity and good separation efficiency for specific steviol glycosides. The present invention is related to a separation medium in which polyethyleneimine is immobilized to porous particles of a (meth)acrylic polymer having a crosslinked structure and a hydroxyl group.
Claims18
1-8. (canceled)
A separation method for steviol glycosides, the method comprising a liquid chromatography step of loading a solution containing two or more types of steviol glycosides to a separation medium and flowing a solvent A through the separation medium, thereby separating at least two types of steviol glycosides included in the steviol glycosides, wherein the separation medium comprises polyethyleneimine immobilized to porous particles of a (meth)acrylic polymer having a crosslinked structure and a hydroxyl group.
A separation method for steviol glycosides, the method comprising an adsorption step of bringing a solution containing two or more types of steviol glycosides and a solvent B into contact with a separation medium to form a steviol glycosides adsorbed separation medium, and an elution step of eluting the steviol glycosides from the steviol glycosides adsorbed separation medium by using a solvent C, thereby obtaining two or more fractions, each containing different steviol glycosides as a main component.
A separation method for steviol glycosides, wherein a separation medium in which polyethyleneimine is immobilized to porous particles of a (meth)acrylic polymer is used for separation of the steviol glycoside.
Dependencies are inferred from the claim text.