Devices, Systems, and Methods of Electronic Modulation of Polymerase for DNA Synthesis
AssigneeGeorgia Tech Research Corporation
InventorsHua Wang, Andrew Hessel
Life of this patent
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Abstract
A method of synthesis of a nucleotide chain, the nucleotide chain including an ordered plurality of nucleotides, the method including: identifying a first nucleotide of the ordered plurality of nucleotides; controlling a polymerase enzyme to assemble the first nucleotide onto the nucleotide chain by electrically modulating an electrode; identifying a subsequent nucleotide in the ordered plurality of nucleotides as a current nucleotide; and controlling the polymerase enzyme to assemble the current nucleotide onto an end of the nucleotide chain by electrically modulating the electrode.
Claims23
A method of synthesis of a nucleotide chain, the nucleotide chain including an ordered plurality of nucleotides, the method comprising:
- identifying a first nucleotide of the ordered plurality of nucleotides;
- controlling a polymerase enzyme to assemble the first nucleotide onto the nucleotide chain by electrically modulating an electrode;
- identifying a subsequent nucleotide in the ordered plurality of nucleotides as a current nucleotide;
- and
- controlling the polymerase enzyme to assemble the current nucleotide onto an end of the nucleotide chain by electrically modulating the electrode.
A system comprising:
- an electrode;
- a polymerase enzyme immobilized adjacent to the electrode;
- and
- a processor;
- and
- a memory having stored thereon instructions that, when executed by the processor, control the processor to electrically modulate the electrode to control the polymerase enzyme to assemble a nucleotide chain, the nucleotide chain comprising an ordered plurality of nucleotides.
Dependencies are inferred from the claim text.