Personalized leukemia/lymphoma therapeutic model
AssigneeThe University of Chicago
InventorsY. Lynn Wang, Pin Lu
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Abstract
The disclosure is directed to an ex vivo cell culture system and methods of using the cell culture system to identify potential therapeutic agents for the treatment of leukemia or lymphoma, such as chronic lymphocytic leukemia (CLL). The ex vivo culture system comprises (a) a first cell culture comprising bone marrow stromal cells (BMSC) which express one or more exogenous cell signaling molecules; (b) a second cell culture comprising leukemia or lymphoma cells isolated from a human; and optionally (c) one or more soluble cell signaling molecules.
Claims25
An ex vivo cell culture system, which comprises (a) a first cell culture comprising bone marrow stromal cells (BMSC) which express one or more exogenous cell signaling molecules;
- (b) a second cell culture comprising leukemia or lymphoma cells isolated from a human;
- and optionally (c) one or more soluble cell signaling molecules.
A method of treating leukemia or lymphoma in a subject in need thereof, which method comprises:
- (a) isolating leukemia or lymphoma cells from the subject,
- (b) contacting an ex vivo cell culture system comprising BMSCs expressing an exogenous signaling molecule with the isolated leukemia or lymphoma cells and optionally one or more soluble cell signaling molecules;
- (c) treating the ex vivo cell culture system with at least one candidate therapeutic agent;
- (d) measuring one or more of survival, proliferation, adhesion, and/or migration of the leukemia or lymphoma cells following step (c), wherein a decrease in survival, proliferation, adhesion, and/or migration of the leukemia or lymphoma cells as compared to cells not treated with the at least one candidate therapeutic agent indicates that the at least one candidate therapeutic agent inhibits the leukemia or lymphoma;
- and
- (e) administering the at least one candidate therapeutic agent to the subject, whereby the leukemia or lymphoma is treated.
Dependencies are inferred from the claim text.