Neural stem cell compositions and methods to treat neurodegenerative disorders
AssigneeThe Regents of the University of California
InventorsJohn REIDLING, Brian FURY, Leslie Michels THOMPSON, Gerhard Bauer, Dane COLEAL-BERGUM
Life of this patent
Estimating the term…
Abstract
Provided herein are stem-cell based therapies for the treatment of neurodegenerative diseases and CNS disorder such as Huntington's disease. The therapy improved motor deficits and rescued synaptic alterations. The cells were shown to be electrophysiologically active and that they improved motor and late-stage cognitive impairment.
Claims28
A method to prepare a human neuronal stem cell (hNSC) from a human embryonic stem cell (hESC), the method comprising the steps of:
- a) isolating at least one stem cell rosette from a population of embryoid bodies (EB) cultured in differentiation medium;
- b) culturing at least one individual cell isolated from the rosette of step a) for an amount of time and under until conditions that provide for the generation of at least one rosette;
- c) isolating an individual cell from the rosette of step b) into individual cells;
- and
- d) culturing the at least one individual cell isolated from step c) for an amount of time and under until conditions that provide for the generation of confluent population of hNSCs.
3-9. (canceled)
(canceled)
(canceled)
(canceled)
(canceled)
28-29. (canceled)
31-33. (canceled)
(canceled)
Dependencies are inferred from the claim text.