Microfluidic device for detecting biomolecules in sweat and wearable biosensor patch using the same

A skin-attachment-type biosensor patch to detect a biomolecule in sweat, the biosensor patch comprising:

• a fluidic passing layer comprising
• an inlet part configured to collect sweat secreted from the skin,
• a sensing element in communication with the inlet part via a first microchannel and being configured to receive sweat from the inlet part,
• a reagent storage part in communication with the sensing element part via a second microchannel and being configured to supply a detection reagent to the sensing element part, and
• a disposer in communication with the sensing element part via a third microchannel and being configured to accommodate sweat of the sensing element part;
• and
• a fluidic connection layer comprising
• a microfluidic tube arranged in a vertical direction to supply sweat secreted from the skin to the inlet part,
• a sensing space provided between the sensing element and a biosensor to transfer sweat of the sensing element to the biosensor, and
• a reagent storage space positioned below the reagent storage part to store the detection reagent,
• wherein the fluidic connection layer is disposed below the fluidic passing layer, and the biosensor is provided below the fluidic connection layer and comprises a probe configured to detect the biomolecule.

A method of manufacturing a microfluidic device, the method comprising:

• fabricating a three-dimensional mold for manufacturing a microfluidic device using a 3D printer;
• forming a fluidic passing layer and a fluidic connection layer by pouring an elastic polymer solution into the mold and thermally curing the solution;
• treating the fluidic passing layer and the fluidic connection layer with a hydrophilic polymer;
• and
• combining the fluidic passing layer and the fluidic connection layer, subsequent to the treating with the hydrophilic polymer.