Generation of human endodermal organs in pig model using lineage restricted endodermal precursors
AssigneeUniversity of Maryland, College Park
InventorsChi-Hun Park, Bhanu Prakash V.L. Telugu
Life of this patent
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Abstract
Disclosed are methods of growing and culturing xenotypic tissue or xenotypic organs in a mammalian species. The methods of growing human organs in other mammalian species are disclosed and such human organs can be used for transplant purposes.
Claims27
A method of creating xenotypic organ cells in an animal comprising:
- (a) contacting a gene-modifying amino acid sequence and/or gene-modifying nucleic acid sequence with one or a plurality of XEN cells from a first species or one or a plurality of embryos from a second species for a time period sufficient to produce a genetic modification in a genome of the one or a plurality of XEN cells or the one or a plurality of embryos;
- (b) injecting the one or a plurality of XEN cells from one species into the one or a plurality of embryos;
- (c) implanting the embryo into a female host from the second species to produce a genetically modified fetus.
A method of producing a germline mutation in the endodermal or mesodermal tissue of a fetus, the method comprising:
- (a) injecting one or a plurality of XEN cells and/or XEN-like cells from a first species into an embryo of a second species;
- and
- (b) contacting a gene-modifying amino acid sequence and/or gene-modifying nucleic acid sequence with one or a plurality of XEN cells and/or XEN-like cells;
- or
- contacting a gene-modifying amino acid sequence and/or gene-modifying nucleic acid sequence with one or a plurality of embryos.
9-11. (canceled)
(canceled)
A method of growing a xenotypic organ or organ tissue in an animal comprising:
- (a) contacting a gene-modifying amino acid sequence and/or gene-modifying nucleic acid sequence with one or a plurality of mammalian embryos from one species for a time period sufficient to produce a genetic modification in a genome of the one or a plurality of embryos;
- and
- (b) injecting one or a plurality of XEN cells from a second species into an embryo of the first species.
(canceled)
(canceled)
(canceled)
A method of microinjecting XEN cells and/or XEN-like cells from a first mammalian species into an embryo of a second mammalian species comprising:
- (a) harvesting XEN cells and/or XEN-like cells from a culture;
- (b) culturing the embryo;
- and
- (c) injecting the XEN cells and/or XEN-like cells into the embryo.
(canceled)
29-31. (canceled)
Dependencies are inferred from the claim text.