Systems, methods and kits for micropropagation of cannabis
AssigneeFront Range Biosciences, Inc.
InventorsCarmen Cecilia ZAPATA CARRERO, Carolina SARMIENTO, Jonathan David VAUGHT
Life of this patent
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Abstract
Provided herein are systems, methods and kits for micropropagating disease-free cannabis plants, referred to as the Clean Stock® method. Also provided are Clean Stock® cannabis plants produced using such systems, methods and kits.
Claims22
A system for in vitro propagation of disease-free clonal cannabis plants, comprising:
- a clean explant from a mother cannabis plant maintained in a vegetative state throughout its life, wherein the mother cannabis plant is a virus-tested and culture-indexed mother cannabis plant;
- aseptic tissue culture media for propagation of the clean explant, wherein the tissue culture media comprises Driver and Kuniyaki Walnut (DKW) media;
- an incubator for propagation of the clean explant comprising conditions effective to produce disease-free cannabis plants;
- and
- disease-free clonal cannabis plants.
(canceled)
A method for in vitro propagation of disease-free clonal cannabis plants, comprising:
- obtaining a clean explant from a mother cannabis plant;
- placing the clean explant on tissue culture media under aseptic conditions on day Q wherein the tissue culture media comprises Driver and Kuniyaki Walnut (DKW) media;
- and
- micropropagating the explant to produce disease-free clonal cannabis plants, wherein within 15 to 17 weeks following day 0, the number of clonal disease-free cannabis plants produced is at least 200 times (200×), at least 210 times (210×), at least 220 times (220×), at least 230 times (230×), at least 240 times (240×), at least 250 times (250×), or at least at least 256 times (256×) the number of starting clean explants, or
- micropropagating the clean explant to produce disease-free clonal cannabis plants, wherein within 15 to 17 weeks following day 0, the number of clonal disease-free cannabis plants produced is at least 1000 times (1000×), at least 2000 times (2000×), at least 3000 times (3000×), at least 4000 times (4000×), at least 4020 times (4020×), at least 4040 times (4040×), at least 4060 times (4060×), at least 4080 times (4080×), or at least 4096 times (4096×) the number of starting clean explants.
11-13. (canceled)
15-20. (canceled)
A method of producing true-to-type disease-free clonal cannabis plants by micropropagation of an explant from a mother cannabis plant, comprising:
- (a) initiating tissue culture from an explant of a mother cannabis plant;
- (b) performing culture indexing;
- (c) micropropagating the explant;
- (d) pre-rooting or rooting the explant;
- and
- (e) acclimatizing the explant, wherein the method is effective to produce a number of disease-free clonal cannabis plants that is at least 200 times (200×) the number of starting explants within 15 to 17 weeks of initiation of the culture, and wherein the method comprises culturing the explants with tissue culture media comprising Driver and Kuniyaki Walnut (DKW) media.
(canceled)
26-28. (canceled)
Dependencies are inferred from the claim text.