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US 2021/0222209 A1Published application
Enzymatic methods for butanol production
AssigneeNewpek S.A. De C.V.
InventorsClaudio Garibay ORIJEL, Monica Maria Rios LOZANO, Jessica Valeria Guerrero TORRES, Ivan Alejandro de la Pen MIRELES, Jose Raul Ivan Garza RODRIGUEZ
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Abstract
A process for producing butanol is provided, involving: A) mixing water, lactate, an enzyme mixture comprising at least one enzyme, at least one cofactor and at least one coenzyme, to prepare a reaction mixture; B) catalytically reacting the reaction mixture for an amount of time sufficient to cause conversion of lactate into butanol; and wherein the conversion of lactate into butanol in B) is associated with a regeneration system of NAD (P)+/NAD (P) H and/or acetyl-CoA/CoA.
Claims25
1:
- A process for producing butanol, comprising:
- A) mixing water, lactate, an enzyme mixture comprising at least one enzyme, at least one cofactor and at least one coenzyme, to prepare a reaction mixture;
- B) catalytically reacting the reaction mixture for an amount of time sufficient to cause conversion of lactate into butanol;
- and
- C) separating the butanol from a reactant obtained by the catalytic conversions in B);
- wherein the conversion of lactate into butanol in B) is associated with a Nicotinamide Adenine Dinucleotide (NAD+) and Reduced Nicotinamide Adenine Dinucleotide (NADH) and/or Nicotinamide adenine dinucleotide phosphate (NADP+) and Reduced Nicotinamide adenine dinucleotide phosphate (NADPH) regenerating system.
3 more elements
(canceled)
3:
- The process of claim 1 wherein, in B):
- the lactate is converted into pyruvate;
- the pyruvate is converted into acetyl-CoA;
- the acetyl-CoA is converted into acetoacetyl-CoA;
- the acetoacetyl-CoA is converted into 3-hydroxybutyryl-CoA;
- the 3-hydroxybutyryl-CoA is converted into crotonyl-CoA;
- the crotonyl-CoA is converted into butyryl-CoA;
- the butyryl-CoA is converted into butanal;
- and
- the butanal is converted into butanol.
7 more elements
4:
- The process of claim 1 wherein, in B):
- the lactate is converted into pyruvate;
- the pyruvate is converted to acetyl-CoA and formate;
- the formate is converted into CO2;
- the acetyl-CoA is converted into acetoacetyl-CoA;
- the acetoacetyl-CoA is converted into 3-hydroxybutyryl-CoA;
- the 3-hydroxybutyryl-CoA is converted into butyryl-CoA;
- the butyryl-CoA is converted into butanal;
- and
- the butanal is converted into butanol.
7 more elements
5:
- The process of claim 1 wherein, in B):
- the lactate is converted into pyruvate;
- the pyruvate is converted into acetolactate;
- the acetolactate is converted into 2,3-dihydroxyvalerate;
- the 2,3-dihydroxyvalerate is converted into ketoisovalerate;
- the ketoisovalerate is converted into isobutyryl-CoA;
- the isobutyryl-CoA is converted into butyryl-CoA;
- the butyryl-CoA is converted into butanal;
- and
- the butanal is converted into butanol.
7 more elements
6:
- The process of claim 1 wherein the conversion efficiency of butanol from lactate is 98 to 100%.
7:
- The process of claim 1 wherein:
- (i) the enzyme mixture is prepared in a container before mixing in A);
- or
- (ii) wherein the enzyme mixture is prepared in a container before mixing in A), wherein the container is a tube, a tank or a reactor or a combination thereof;
- or
- (iii) wherein in A), the mixing is carried out in a suitable container to prepare the reaction mixture;
- or
- (iv) wherein in A), the mixing is carried out in a suitable container to prepare the reaction mixture, wherein the suitable container is a tube, a tank, a reactor, or a combination thereof;
- or
- (v) wherein in A), the mixing is carried out by an appropriate method to allow interaction between at least one enzyme and the substrates and/or compounds;
- or
- (vi) wherein in A), the mixing is carried out mechanically, pneumatically, hydraulically or by a combination thereof;
- or
- (vii) wherein, in B), the catalytic conversions are carried out in a container or a reactor suitable to promote interactions between enzymes and substrates and/or compounds;
- or
- (viii) wherein, in B), the catalytic conversions are carried out in a container or a reactor suitable to promote interactions between enzymes and substrates and/or compounds, wherein the container or reactor is a stirred tank, a plug flow reactor, a fluidized bed reactor, a packed bed reactor, or any combination thereof;
- or
- (ix) wherein, in B), the catalytic conversions are carried out in a pH range of 5 to 10.
15 more elements
8-18. (canceled)
19:
- The process of claim 1 wherein the process is carried out continuously, semicontinuously or batch manner.
20:
- The process of claim 1 wherein the lactate is L-lactate, D-lactate or a mixture of both.
21:
- The process of claim 1 wherein the lactate concentration in the reaction mixture is at least 1 g/L.
22:
- The process of claim 1 wherein the enzyme mixture contains at least one enzyme selected from the group consisting of:
- lactate dehydrogenase (EC 1.1.1.27 and/or EC 1.1.1.28), pyruvate dehydrogenase (EC 1.2.1.51, EC 1.2.4.1, EC 2.3.1.12 and/or EC 1.8.1.4), acetoacetyl-CoA thiolase (EC 2.3.1.9), 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.35 and/or EC 1.1.1.157), enoyl-CoA hydratase (EC 4.1.1.55 and/or EC 4.2.1.17), butyryl-CoA dehydrogenase (EC 1.3.1.44, EC 1.3.1.86 and/or 1.3.8.1), butanal dehydrogenase (EC 1.2.1.57 and/or EC 1.2.1.10), alcohol dehydrogenase (EC 1.1.1.1 and/or EC 1.1.1.2) pyruvate formate lyase (EC 2.3.1.54), formate dehydrogenase (EC 1.2.1.43), acetolactate synthase (EC 2.2.1.6), ketoacid reductoisomerase (EC 1.1.1.86), dihydroxy acid dehydratase (EC 4.2.1.9), 2-oxoisovalerato dehydrogenase (EC 1.2.1.25 and/or EC 1.2.4.4), isobutyryl-CoA mutase (EC 5.4.99.13) or its analogs.
23:
- The process of claim 1 wherein at least one enzyme may be immobilized, entrapped, embedded, adhered, attached, secured, absorbed, adsorbed or otherwise attached or associated with a support.
24:
- The process of claim 23 wherein the support is at least one of the following types of media, zeolite, activated carbon, acrylamide, agarose, silica gel, silica, alginate or methacrylate polymers.
25:
- The process of claim 1 wherein the concentration of enzymes in the enzyme mixture is higher than 0.001 g/L.
26:
- The process of claim 23 wherein the concentration of enzymes on the support is greater than 0.001 grams of enzyme per gram of support.
27:
- The process of claim 1 wherein the amount of NAD (P)+/NAD (P) H and/or the amount of acetyl CoA/CoA in the regeneration system is less than stoichiometrically required.
(canceled)
29:
- The process of claim 1 wherein the total amount of NAD (P)+ and NAD (P) H and/or the total amount of acetyl CoA and CoA used to convert two moles lactate in a mole of isobutanol is less than 1 mol.
(canceled)
31:
- The process of claim 1 wherein the separation of butanol from the reaction mixture comprises:
- C-1) separating the reactants obtained by the catalytic conversions in B) into a first stream and a second stream, wherein the first stream comprises butanol and water, and the second stream comprises components in the reactants different from butanol.
32:
- The process of claim 31, wherein the second stream is recycled or reused by mixing it with the reaction mixture in A), B), or both.
33:
- The process of claim 31, wherein, in C-1), the separation is carried out by an appropriate method to separate based on their physicochemical properties molecules.
34:
- The process of claim 31, wherein the separation is carried out by membranes, distillation, evaporation, or a combination of any of the above.
35-39. (canceled)
Dependencies are inferred from the claim text.