Compositions and methods for increasing extractability of solids from coffee beans
AssigneeTropic Biosciences UK Limited
InventorsEyal MAORI, Yaron GALANTY, Cristina PIGNOCCHI, Angela CHAPARRO GARCIA, Ofir MEIR
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Abstract
A coffee plant comprising a genome comprising a loss of function mutation in a nucleic acid sequence encoding alpha-D-galactosidase. Also provided is a method of increasing extractability of solids from coffee beans. In addition there is provided a method of producing soluble coffee.
Claims27
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A method of increasing extractability of solids from coffee beans, the method comprising:
- (a) subjecting a coffee plant cell to a DNA editing agent directed at a nucleic acid sequence encoding alpha-D-galactosidase to result in a loss of function mutation in said nucleic acid sequence encoding said alpha-D-galactosidase;
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- (b) regenerating a plant from said plant cell.
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A nucleic acid construct comprising a nucleic acid sequence encoding a DNA editing agent directed at coffee alpha-D-galactosidase being operably linked to a plant promoter.
A coffee plant, or a plant part thereof, comprising a genome comprising a loss of function mutation in a nucleic acid sequence encoding alpha-D-galactosidase, wherein the plant is non-transgenic, optionally wherein the plant part is a bean.
Dependencies are inferred from the claim text.