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US 2021/0222136 A1Published application
Ectoine-producing yeast
AssigneeAlderys
InventorsDominique LOUIS, Karine JAILLARDON, Dominique THOMAS
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Abstract
The present invention relates to the field of bio-production of ectoine. There is a need in the art for ectoine production methods allowing its highly efficient synthesis and secretion. The solution proposed in the present invention is the use of a genetically modified yeast comprising many modifications as described in the present text.
Claims15
An ectoine-producing recombinant yeast, in the genome of which:
- (A) (i) at least one nucleic acid encoding an aspartokinase is overexpressed and/or is under the control of an inducible or repressible promoter;
- and/or
- (ii) at least one nucleic acid encoding an aspartate kinase is overexpressed and/or is under the control of an inducible or repressible promoter;
- (B) at least one nucleic acid encoding an aspartate semi-aldehyde dehydrogenase and/or at least one nucleic acid encoding an aspartate semi-aldehyde dehydrogenase that can use as coenzyme both NAD and NADP is overexpressed and/or is under the control of an inducible or repressible promoter;
- (C) at least one nucleic acid encoding a diaminobutyrate aminotransferase is overexpressed and/or is under the control of an inducible or repressible promoter;
- (D) (i) at least one nucleic acid encoding an homoserine-O-acetyltransferase MET2 is overexpressed and/or is under the control of an inducible or repressible promoter;
- (ii) at least one nucleic acid encoding an homoserine-O-acetyltransferase METX is overexpressed and/or is under the control of an inducible or repressible promoter, and/or
- (iii) at least one nucleic acid encoding a diaminobutyric acid acetyltransferase is overexpressed and/or is under the control of an inducible or repressible promoter;
- (E) at least one nucleic acid encoding an ectoine synthase is overexpressed and/or is under the control of an inducible or repressible promoter;
- (F) (i) at least one endogenous nucleic acid encoding an homoserine dehydrogenase has been deleted and/or interrupted, and/or
- (ii) at least one nucleic acid encoding an homoserine dehydrogenase is independently:
- under the control of an inducible or repressible promoter;
- under the control of a weak promoter;
- and/or
- in a destabilized form.
12 more elements
(canceled)
Dependencies are inferred from the claim text.